Publications of Cornelius Krasel

Original contributions

  1. P. M. Burger, J. Hell, E. Mehl, C. Krasel, F. Lottspeich, R. Jahn:
    GABA and glycine in synaptic vesicles: storage and transport characteristics.
    Neuron 7, 287-293 (1991)
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  2. A. M. Gabilondo, C. Krasel, M. J. Lohse:
    Mutations of Tyr-326 in the β2-adrenoceptor disrupt multiple receptor functions.
    Eur. J. Pharmacol. 307, 243-250 (1996)
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  3. R. Winstel, S. Freund, C. Krasel, E. Hoppe, M. J. Lohse:
    Protein kinase cross-talk: membrane targeting of the β-adrenergic receptor kinase by protein kinase C.
    Proc. Natl. Acad. Sci. USA 93, 2105-2109 (1996)
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  4. K. Wieland, H. M. Zuurmond, C. Krasel, A. P. IJzerman, M. J. Lohse:
    Involvement of Asn-293 in stereospecific agonist recognition and in activation of the β2-adrenergic receptor.
    Proc. Natl. Acad. Sci. USA 93, 9276-9281 (1996)
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  5. A. M. Gabilondo, J. Hegler, C. Krasel, V. Boivin-Jahns, L. Hein, M. J. Lohse:
    A di-leucine motif in the C-terminus of the β2-adrenergic receptor promotes receptor internalization.
    Proc. Natl. Acad. Sci. USA 94, 12285-12290 (1997)
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  6. D. A. Groarke, S. Wilson, C. Krasel, G. Milligan:
    Visualization of agonist-induced association and trafficking of green fluorescent protein-tagged forms of both β-arrestin-1 and the thyrotropin-releasing hormone receptor-1.
    J. Biol. Chem. 274, 23263-23269 (1999)
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  7. C. Krasel, S. Dammeier, R. Winstel, J. Brockmann, H. Mischak, M. J. Lohse:
    Phosphorylation of GRK2 by protein kinase C abolishes its inhibition by calmodulin.
    J. Biol. Chem. 276, 1911-1915 (2001)
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  8. J.-P. Vilardaga, M. Frank, C. Krasel, C. Dees, R. A. Nissenson, M. J. Lohse:
    Differential conformational requirements for activation of G proteins and regulatory proteins, arrestin and GRK in the G protein-coupled receptor for parathyroid hormone (PTH)/PTH related protein.
    J. Biol. Chem. 276, 33435-33443 (2001)
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  9. J.-P. Vilardaga*, C. Krasel*, S. Chauvin, T. Bambino, M. J. Lohse, R. A. Nissenson: (*: joint author)
    Internalization determinants of the parathyroid hormone receptor differentially regulate β-arrestin/receptor association.
    J. Biol. Chem. 277, 8121-8129 (2002)
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  10. M. Castro, F. Dicker, J.-P. Vilardaga, C. Krasel, M. Bernhardt, M. J. Lohse:
    Dual regulation of the parathyroid hormone (PTH)/PTH-related peptide receptor signaling by protein kinase C and β-arrestins.
    Endocrinol. 143, 3854-3865 (2002)
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  11. A. Hannawacker, C. Krasel, M. J. Lohse:
    Mutation of Asn293 to Asp in transmembrane helix VI abolishes agonist-induced but not constitutive activity of the β2-adrenergic receptor.
    Mol. Pharmacol. 62, 1431-1437 (2002)
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  12. T. Eichmann, K. Lorenz, M. Hoffmann, J. Brockmann, C. Krasel, M. J. Lohse, U. Quitterer:
    The amino-terminal domain of G-protein-coupled receptor kinase 2 is a regulatory Gβγ binding site.
    J. Biol. Chem. 278, 8052-8057 (2003)
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  13. J.-P. Vilardaga, M. Bünemann, C. Krasel, M. Castro, M. J. Lohse:
    Measurement of the millisecond activation switch of G protein-coupled receptors in living cells.
    Nature Biotechnol. 21, 807-812 (2003)
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  14. C. Krasel, M. Bünemann, K. Lorenz, M. J. Lohse:
    β-Arrestin binding to the β2-adrenergic receptor requires both receptor phosphorylation and receptor activation.
    J. Biol. Chem. 280, 9528-9535 (2005)
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  15. R. Winstel, H.-G. Ihlenfeldt, G. Jung, C. Krasel, M. J. Lohse:
    Peptide inhibitors of G protein-coupled receptor kinases.
    Biochem. Pharmacol. 70, 1001-1008 (2005)
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  16. R. Steinmeyer*, A. Noskov*, C. Krasel*, I. Weber*, C. Dees, G. S. Harms: (*: equal authorship)
    Improved fluorescent proteins for single-molecule research in molecular tracking and co-localization.
    J. Fluoresc. 15, 707-721 (2005)
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  17. V. L. Harvey, I. C. Duguid, C. Krasel, G. J. Stephens:
    Evidence that GABA ρ subunits contribute to functional ionotropic GABA receptors in mouse cerebellar Purkinje cells.
    J. Physiol. 577, 127-139 (2006)
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  18. G. Mayer, B. Wulffen, C. Huber, J. Brockmann, B. Flicke, L. Neumann, D. Hafenbradl, B. M. Kleb, M. J. Lohse, C. Krasel, M. Blind:
    An RNA molecule that specifically inhibits G-protein-coupled receptor kinase 2 in vitro.
    RNA 14, 524-534 (2008)
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  19. C. Hoffmann, N. Ziegler, S. Reiner, C. Krasel, M. J. Lohse:
    Agonist-selective, receptor-specific interaction of human P2Y receptors with β-arrestin-1 and -2.
    J. Biol. Chem. 283, 30933-30941 (2008)
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  20. C. Krasel, U. Zabel, K. Lorenz, S. Reiner, S. Al-Sabah, M. J. Lohse:
    Dual role of the β2-adrenergic receptor C-terminus for the binding of β-arrestin and receptor internalization.
    J. Biol. Chem. 283, 31840-31848 (2008)
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  21. J. McPherson, G. Rivero, M. Baptist, J. Llorente, S. Al-Sabah, C. Krasel, W. L. Dewey, C. P. Bailey, E. M. Rosethorne, S. J. Charlton, G. Henderson, E. Kelly:
    μ-Opioid receptors: correlation of agonist efficacy for signalling with ability to activate internalization.
    Mol. Pharmacol. 78, 756-766 (2010)
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  22. G. Cheng, C. Krasel, H. G. Zhou, D. Chappell, I. W. Hamley:
    Genetic expression of an amyloid peptide fragment and analysis of formylated products.
    Org. Lett. 13, 2572-2575 (2011)
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  23. D. Markovic, J. Holdich, S. Al-Sabah, R. Mistry, C. Krasel, M. P. Mahaut-Smith, R. A. J. Challiss:
    FRET-based detection of M1 muscarinic acetylcholine receptor activation by orthorsteric and allosteric agonists.
    PLoS ONE 7, e29946 (2012)
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  24. G. Rivero, J. Llorente, J. McPherson, A. Cooke, S. J. Mundell, C. A. McArdle, E. M. Rosethorne, S. J. Charlton, C. Krasel, C. P. Bailey, G. Henderson, E. Kelly:
    Endomorphin-2: a biased agonist at the μ opioid receptor.
    Mol. Pharmacol. 82, 178-188 (2012)
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  25. M. Lampe, F. Pierre, S. Al-Sabah, C. Krasel, C. J. Merrifield:
    Dual single scission event analysis of constitutive transferrin receptor (TfR) endocytosis and ligand-triggered β2-adrenergic receptor (β2AR) or μ-opioid receptor (MOR) endocytosis.
    Mol. Biol. Cell 25, 3070-3080 (2014)
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  26. S. Al-Sabah, M. Al-Fulaij, G. Shaaban, H. A. Ahmed, D. Donnelly, M. Bünemann, C. Krasel:
    The GIP receptor displays higher basal activity than the GLP-1 receptor but does not recruit GRK2 or arrestin3 effectively.
    PLoS One 9: e106890 (2014)
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  27. A. E. Cooke, S. Oldfield, C. Krasel, S. J. Mundell, G. Henderson, E. Kelly:
    Morphine-induced internalization of the L83I mutant of the rat μ-opioid receptor.
    Br. J. Pharmacol. 172: 594-605 (2015)
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  28. V. Wolters, C. Krasel, J. Brockmann, M. Bünemann:
    Influence of Gαq on the dynamics of M3-acetylcholine receptor-G-protein-coupled receptor kinase 2 interaction.
    Mol. Pharmacol. 87: 9-17 (2015)
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  29. D. Zindel, A. J. Butcher, S. Al-Sabah, P. Lanzerstorfer, J. Weghuber, A. B. Tobin, M. Bünemann, C. Krasel:
    Engineered hyperphosphorylation of the β2-adrenoceptor prolongs arrestin-3 binding and induces arrestin internalization.
    Mol. Pharmacol. 87: 349-362 (2015)
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  30. D. Zindel, S. Engel, A. R. Bottrill, J.-P. Pin, L. Prézeau, A. B. Tobin, M. Bünemann, C. Krasel*, A. J. Butcher*: (*: equal authorship)
    Identification of key phosphorylation sites in PTH1R which determine arrestin3 binding and fine tune receptor signaling.
    Biochem. J. 473: 4173-4192 (2016)
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  31. T. Sun, L. Yang, H. Kaur, J. Pestel, M. Looso, H. Nolte, C. Krasel, D. Heil, R. K. Krishnan, M.-J. Santoni, J.-P. Borg, M. Bünemann, S. Offermanns, J. M. Swiercz, T. Worzfeld:
    A reverse signaling pathway downstream of Sema4A controls cell migration via Scrib.
    J. Cell Biol. 216: 199-215 (2017)
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  32. B. Honrath, L. Matschke, T. Meyer, L. Magerhans, F. Perocchi, G. K. Ganjam, H. Zischka, C. Krasel, A. Gerding, B. M. Bakker, M. Bünemann, S. Strack, N. Decher, C. Culmsee, A. Dolga:
    SK2 channels regulate mitochondrial respiration and mitochondrial Ca2+ uptake.
    Cell Death Differ. 24: 761-773 (2017)
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  33. E. Miess, A. B. Gondin, A. Yousuf, R. Steinborn, N. Mösslein, Y. Yang, M. Göldner, J. G. Ruland, M. Bünemann, C. Krasel, M. J. Christie, M. L. Halls, S. Schulz, M. Canals:
    Multisite phosphorylation is required for sustained interaction with GRKs and arrestins during rapid μ-opioid receptor desensitization.
    Sci. Signal. 11: eeas9609 (2018)
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  34. N. Al-Zamel, S. Al-Sabah, Y. Luqmani, L. Adi, S. Chacko, T. D. Schneider, C. Krasel:
    A dual GLP-1/GIP receptor agonist does not antagonize glucagon at its receptor but may act as a biased agonist at the GLP-1 receptor.
    Int. J. Mol. Sci. 20: E3532 (2019)
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    Correction in Int. J. Mol. Sci. 21: 3357 (2020)
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  35. T. Maimari, C. Krasel, M. Bünemann, K. Lorenz:
    The N-termini of GRK2 and GRK3 simulate the stimulating effects of RKIP on β-adrenoceptors.
    Biochem. Biophys. Res. Commun. 520: 327-332 (2019)
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  36. A. Gillis, A. B. Gondin, A. Kliewer, J. Sanchez, H. D. Lim, C. Alamein, P. Manandhar, M. Santiago, S. Fritzwanker, F. Schmidel, T. A. Katte, T. Reekie, N. L. Grimsey, M. Kassiou, B. Kellam, C. Krasel, M. L. Halls, M. Connor, J. R. Lane, S. Schulz, M. J. Christie, M. Canals:
    Low intrinsic efficacy for G protein activation can explain the improved side effect profiles of new opioid agonists.
    Sci. Signal. 13: eaaz3140 (2020)
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  37. S. Al-Sabah, L. Adi, M. Bünemann, C. Krasel:
    The effect of cell surface expression and linker sequence on the recruitment of arrestin to the GIP receptor.
    Front. Pharmacol. 11: 1271 (2020).
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  38. A. Flöser, K. Becker, E. Kostenis, G. König, C. Krasel, P. Kolb, M. Bünemann:
    Disentangling bias between Gq, GRK2, and arrestin3 recruitment to the M3 muscarinic acetylcholine receptor.
    ELife 10: e58442 (2021).
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  39. N. Mösslein, L.-M. G. Pohle, A. Fuss, M. Bünemann, C. Krasel:
    Residency time of agonists does not affect the stability of GPCR-arrestin complexes.
    Br. J. Pharmacol. 179: 4107-4116 (2022).
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  40. R. S. Haider, E. S. F. Matthees, J. Drube, M. Reichel, U. Zabel, A. Inoue, A. Chevigné, C. Krasel, X. Deupi, C. Hoffmann:
    β-arrestin1 and 2 exhibit distinct phosphorylation-dependent conformations when coupling to the same GPCR in living cells.
    Nat. Commun. 13: 5638 (2022).
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  41. B. Al-Zaid, S. Chacko, C. I. Ezeamuzie, M. Bünemann, C. Krasel, T. Karimian, P. Lanzerstorfer, S. Al-Sabah:
    Differential effects of glucose-dependent insulinotropic polypeptide receptor/glucagon-like peptide-1 receptor heteromerization on cell signaling when expressed in HEK-293 cells.
    Pharmacol. Res. Perspect. 10: e01013 (2022).
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  42. T. Wang, J. Shao, S. Kumar, M. W. Alnouri, J. Carvalho, S. Günther, C. Krasel, K. T. Murphy, M. Bünemann, S. Offermanns, N. Wettschureck:
    Orphan GPCR GPRC5C facilitates angiotensin II-induced smooth muscle contraction.
    Circ. Res. 134: 1259-1275 (2024).
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  43. S. Rinné, , F. Schick, K. Vowinkel, S. Schütte, C. Krasel, S. Kauferstein, M. K.-H. Schäfer, A. K. Kiper, T. Müller, N. Decher:
    Potassium channel TASK-5 forms functional heterodimers with TASK-1 and TASK-3 to break its silence.
    Nat. Commun. 15: 7548 (2024).
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  44. J. Kwon, H. Kawase, K. Mattonet, S. Guenther, L. Hahnefeld, J. Shamsara, J. Heering, M. Kurz, S. Kirchhofer, C. Krasel, M. Ulrich, M. Persechino, S. Murthy, C. Orlandi, C. D. Sadik, G. Geisslinger, M. Bünemann, P. Kolb, S. Offermanns, N. Wettschureck:
    Orphan G protein-coupled receptor GPRC5B controls macrophage function by facilitating prostaglandin E receptor 2 signaling.
    Nat. Commun. 16: 1448 (2025).
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  45. S. B. Kirchhofer, C. Kurz, L. Geier, A. L. Krett, C. Krasel, M. Bünemann:
    Dynamics of agonist-evoked opioid receptor activation revealed by FRET- and BRET-based opioid receptor conformation sensors.
    Commun. Biol. 8: 198 (2025).
    [ Article ]
     

Reviews

  1. M. J. Lohse, K. Blüml, S. Danner, C. Krasel:
    Regulators of G-protein-mediated signaling.
    Biochem. Soc. Trans. 24, 975-980 (1996)
     
  2. M. J. Lohse, C. Krasel, R. Winstel, F. Mayor:
    G-protein-coupled receptor kinases.
    Kidney Int. 49, 1047-1052 (1996)
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  3. C. Krasel, J.-P. Vilardaga, M. Bünemann, M. J. Lohse:
    The kinetics of G protein-coupled receptor signaling and desensitization.
    Biochem. Soc. Trans. 32, 1029-1031 (2004).
    [ PDF ]
     
  4. C. Krasel, M. Bünemann:
    Opioid receptors: single molecule studies shed light on mechanisms of efficacy.
    Signal Transd. Targ. Ther. 9, 219 (2024).
    [ Article ]
     

Book chapers

  1. C. Krasel, M. J. Lohse:
    Signaling in the β-adrenergic receptor system.
    In: Proc. XIVth Intern. Symp. Med. Chem., F. Awouters (ed.), Elsevier, Amsterdam 1997.
     
  2. M. J. Lohse, C. Krasel:
    G-protein-coupled receptor kinases.
    xPharm (executive editors: S. J. Enna, D. B. Bylund), Elsevier, New York 2004.
     
  3. C. Krasel, C. Hoffmann:
    Using intramolecular fluorescence resonance energy transfer to study receptor conformation.
    Chapter 7 in: D. Poyner, M. Wheatley (eds.): G-protein-coupled receptors.
    Wiley-Blackwell 2010. ISBN 978-0-47-074914-2 (print) 978-0-47.074921-0 (on-line).
    [ PDF ]
     
  4. C. Krasel, A. Rinne, M. Bünemann:
    Investigation of muscarinic receptors by fluorescent techniques.
    Chapter 8 in: J. Myslivecek, J. Jakubik (eds.): Muscarinic receptor: from structure to animal models.
    Neuromethods Vol. 107.
    Humana Press 2015, ISBN 978-1-4939-2857-6 (print) 978-1-4939-2858-3 (eBook).
    [ Book (PDF) ]
     
  5. C. Krasel, M. Bünemann:
    Investigation of muscarinic receptors by fluorescent techniques.
    Chapter 9 in: J. Myslivecek, J. Jakubik (eds.): Muscarinic receptor: from structure to animal models.
    Neuromethods Vol. 211.
    Humana Press 2024, ISBN 978-1-0716-4014-2 (print) 978-1-0716-4015-9 (eBook).
    [ Book (PDF) ]