University of Marburg homepage Laboratory of Cornelius Krasel
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Publications

Original contributions

1991

  1. P. M. Burger, J. Hell, E. Mehl, C. Krasel, F. Lottspeich, R. Jahn:
    GABA and glycine in synaptic vesicles: storage and transport characteristics.
    Neuron 7, 287-293 (1991)
     

1996

  1. A. M. Gabilondo, C. Krasel, M. J. Lohse:
    Mutations of Tyr-326 in the β2-adrenoceptor disrupt multiple receptor functions.
    Eur. J. Pharmacol. 307, 243-250 (1996)
    [ PDF ]
     
  2. R. Winstel, S. Freund, C. Krasel, E. Hoppe, M. J. Lohse:
    Protein kinase cross-talk: membrane targeting of the β-adrenergic receptor kinase by protein kinase C.
    Proc. Natl. Acad. Sci. USA 93, 2105-2109 (1996)
    [ PDF ]
     
  3. K. Wieland, H. M. Zuurmond, C. Krasel, A. P. IJzerman, M. J. Lohse:
    Involvement of Asn-293 in stereospecific agonist recognition and in activation of the β2-adrenergic receptor.
    Proc. Natl. Acad. Sci. USA 93, 9276-9281 (1996)
    [ PDF ]
     

1997

  1. A. M. Gabilondo, J. Hegler, C. Krasel, V. Boivin-Jahns, L. Hein, M. J. Lohse:
    A di-leucine motif in the C-terminus of the β2-adrenergic receptor promotes receptor internalization.
    Proc. Natl. Acad. Sci. USA 94, 12285-12290 (1997)
    [ PDF ]
     

1999

  1. D. A. Groarke, S. Wilson, C. Krasel, G. Milligan:
    Visualization of agonist-induced association and trafficking of green fluorescent protein-tagged forms of both β-arrestin-1 and the thyrotropin-releasing hormone receptor-1.
    J. Biol. Chem. 274, 23263-23269 (1999)
    [ PDF ]
     

2001

  1. C. Krasel, S. Dammeier, R. Winstel, J. Brockmann, H. Mischak, M. J. Lohse:
    Phosphorylation of GRK2 by protein kinase C abolishes its inhibition by calmodulin.
    J. Biol. Chem. 276, 1911-1915 (2001)
    [ PDF ]
     
  2. J.-P. Vilardaga, M. Frank, C. Krasel, C. Dees, R. A. Nissenson, M. J. Lohse:
    Differential conformational requirements for activation of G proteins and regulatory proteins, arrestin and GRK in the G protein-coupled receptor for parathyroid hormone (PTH)/PTH related protein.
    J. Biol. Chem. 276, 33435-33443 (2001)
    [ PDF ]
     

2002

  1. J.-P. Vilardaga*, C. Krasel*, S. Chauvin, T. Bambino, M. J. Lohse, R. A. Nissenson: (*: joint author)
    Internalization determinants of the parathyroid hormone receptor differentially regulate β-arrestin/receptor association.
    J. Biol. Chem. 277, 8121-8129 (2002)
    [ PDF ]
     
  2. M. Castro, F. Dicker, J.-P. Vilardaga, C. Krasel, M. Bernhardt, M. J. Lohse:
    Dual regulation of the parathyroid hormone (PTH)/PTH-related peptide receptor signaling by protein kinase C and β-arrestins.
    Endocrinol. 143, 3854-3865 (2002)
    [ PDF ]
     
  3. A. Hannawacker, C. Krasel, M. J. Lohse:
    Mutation of Asn293 to Asp in transmembrane helix VI abolishes agonist-induced but not constitutive activity of the β2-adrenergic receptor.
    Mol. Pharmacol. 62, 1431-1437 (2002)
    [ PDF ]
     

2003

  1. T. Eichmann, K. Lorenz, M. Hoffmann, J. Brockmann, C. Krasel, M. J. Lohse, U. Quitterer:
    The amino-terminal domain of G-protein-coupled receptor kinase 2 is a regulatory Gβγ binding site.
    J. Biol. Chem. 278, 8052-8057 (2003)
    [ PDF ]
     
  2. J.-P. Vilardaga, M. Bünemann, C. Krasel, M. Castro, M. J. Lohse:
    Measurement of the millisecond activation switch of G protein-coupled receptors in living cells.
    Nature Biotechnol. 21, 807-812 (2003)
    [ PDF ]
     

2005

  1. C. Krasel, M. Bünemann, K. Lorenz, M. J. Lohse:
    β-Arrestin binding to the β2-adrenergic receptor requires both receptor phosphorylation and receptor activation.
    J. Biol. Chem. 280, 9528-9535 (2005)
    [ PDF ]
     
  2. R. Winstel, H.-G. Ihlenfeldt, G. Jung, C. Krasel, M. J. Lohse:
    Peptide inhibitors of G protein-coupled receptor kinases.
    Biochem. Pharmacol. 70, 1001-1008 (2005)
    [ PDF ]
     
  3. R. Steinmeyer*, A. Noskov*, C. Krasel*, I. Weber, C. Dees, G. S. Harms: (*: equal authorship)
    Improved fluorescent proteins for single-molecule research in molecular tracking and co-localization.
    J. Fluoresc. 15, 707-721 (2005)
    [ PDF ]
     

2006

  1. V. L. Harvey, I. C. Duguid, C. Krasel, G. L. Stephens:
    Evidence that GABA ρ subunits contribute to functional ionotropic GABA receptors in mouse cerebellar Purkinje cells.
    J. Physiol. (Lond.) 577, 127-139 (2006)
    [ PDF ]
     

2008

  1. G. Mayer, B. Wulffen, C. Huber, J. Brockmann, B. Flicke, L. Neumann, D. Hafenbradl, B. M. Kleb, M. J. Lohse, C. Krasel, M. Blind:
    A RNA molecule that specifically inhibits G-protein coupled receptor kinase 2 in vitro.
    RNA 14, 524-534 (2008)
    [ PDF ]
     
  2. C. Hoffmann, N. Ziegler, S. Reiner, C. Krasel, M. J. Lohse:
    Agonist-selective, receptor-specific interaction of human P2Y receptors with β-arrestin-1 and -2.
    J. Biol. Chem. 283, 30933-30941 (2008)
    [ PDF ]
     
  3. C. Krasel, U. Zabel, K. Lorenz, S. Reiner, S. Al-Sabah, M. J. Lohse:
    Dual role of the β2-adrenergic receptor C-terminus for the binding of β-arrestin and receptor internalization.
    J. Biol. Chem. 283, 31840-31848 (2008)
    [ PDF ]
     

2010

  1. J. McPherson, G. Rivero, M. Baptist, J. Llorente, S. Al-Sabah, C. Krasel, W. L. Dewey, C. P. Bailey, E. M. Rosethorne, S. J. Charlton, G. Henderson, E. Kelly:
    μ-Opioid receptors: correlation of agonist efficacy for signalling with ability to activate internalization.
    Mol. Pharmacol. 78, 756-766 (2010)
    [ PDF ]
     

2011

  1. G. Cheng, C. Krasel, H. G. Zhou, D. D. Chappell, I. W. Hamley:
    Genetic expression of an amyloid peptide fragment and analysis of formylated products.
    Org. Lett. 13, 2572-2575 (2011)
    [ PDF ]
     

2012

  1. D. Markovic, J. Holdich, S. Al-Sabah, R. Mistry, C. Krasel, M. P. Mahaut-Smith, R. A. J. Challiss:
    FRET-based detection of M1 muscarinic acetylcholine receptor activation by orthorsteric and allosteric agonists.
    PLoS ONE 7, e29946 (2012)
    [ PDF ]
     
  2. G. Rivero, J. Llorente, J. McPherson, A. Cooke, S. J. Mundell, C. A. McArdle, E. M. Rosethorne, S. J. Charlton, C. Krasel, C. P. Bailey, G. Henderson, E. Kelly:
    Endomorphin-2: a biased agonist at the μ opioid receptor.
    Mol. Pharmacol. 82, 178-188 (2012)
    [ PDF ]
      ;

2014

  1. M. Lampe, F. Pierre, S. Al-Sabah, C. Krasel, C. J. Merrifield:
    Dual single scission event analysis of constitutive transferrin receptor (TfR) endocytosis and ligand-triggered β2-adrenergic receptor (β2AR) or μ-opioid receptor (MOR) endocytosis.
    Mol. Biol. Cell 25, 3070-3080 (2014)
    [ PDF ]
     
  2. S. Al-Sabah, M. Al-Fulaij, G. Shaaban, H. A. Ahmed, D. Donnelly, M. Bünemann, C. Krasel:
    The GIP receptor displays higher basal activity than the GLP-1 receptor but does not recruit GRK2 or arrestin3 effectively.
    PLoS One 9, e106890 (2014)
    [ Full text ]
     

2015

  1. A. E. Cooke, S. Oldfield, C. Krasel, S. J. Mundell, G. Henderson, E. Kelly:
    Morphine-induced internalization of the L83I mutant of the rat μ-opioid receptor.
    Br. J. Pharmacol. 172: 594-605 (2015)
    [ PDF ]
     
  2. V. Wolters, C. Krasel, J. Brockmann, M. Bünemann:
    Influence of Gαq on the dynamics of M3-acetylcholine receptor-G-protein-coupled receptor kinase 2 interaction.
    Mol. Pharmacol. 87: 9-17 (2015)
    [ PDF ]
     
  3. D. Zindel, A. J. Butcher, S. Al-Sabah, P. Lanzerstorfer, J. Weghuber, A. B. Tobin, M. Bünemann, C. Krasel:
    Engineered hyperphosphorylation of the β2-adrenoceptor prolongs arrestin-3 binding and induces arrestin internalization.
    Mol. Pharmacol. 87: 349-362 (2015)
    [ PDF ]
     

2016

  1. D. Zindel, S. Engel, A. R. Bottrill, J.-P. Pin, L. Prézeau, A. B. Tobin, M. Bünemann, C. Krasel*, A. J. Butcher*: (*: equal authorship)
    Identification of key phosphorylation sites in PTH1R which determine arrestin3 binding and fine tune receptor signaling.
    Biochem. J. 473: 4173-4192 (2016)
    [ PDF ]
     

2017

  1. T. Sun, L. Yang, H. Kaur, J. Pestel, M. Looso, H. Nolte, C. Krasel, D. Heil, R. K. Krishnan, M.-J. Santoni, J.-P. Borg, M. Bünemann, S. Offermanns, J. M. Swiercz, T. Worzfeld:
    A reverse signaling pathway downstream of Sema4A controls cell migration via Scrib.
    J. Cell Biol. 216: 199-216 (2017)
    [ PDF ]
     
  2. B. Honrath, L. Matschke, T. Meyer, L. Magerhans, F. Perocchi, G. K. Ganjam, H. Zischka, C. Krasel, A. Gerding, B. M. Bakker, M. Bünemann, S. Strack, N. Decher, C. Culmsee, A. Dolga:
    SK2 channels regulate mitochondrial respiration and mitochondrial Ca2+ uptake.
    Cell Death Differ. 24: 761-773 (2017)
    [ PDF ]
     

2018

  1. E. Miess, A. B. Gondin, A. Yousuf, R. Steinborn, N. Mösslein, Y. Yang, M. Göldner, J. G. Ruland, M. Bünemann, C. Krasel, M. J. Christie, M. L. Halls, S. Schulz, M. Canals:
    Multisite phosphorylation is required for sustained interaction with GRKs and arrestins during rapid μ-opioid receptor desensitization.
    Sci. Signal. 11: eeas9609 (2018)
    [ PDF ]
     

2019

  1. N. Al-Zamel, S. Al-Sabah, Y. Luqmani, L. Adi, S. Chacko, T. D. Schneider, C. Krasel:
    A dual GLP-1/GIP receptor agonist does not antagonize glucagon at its receptor but may act as a biased agonist at the GLP-1 receptor.
    Int. J. Mol. Sci. 20: E3532 (2019)
    [ PDF ]
     
  2. T. Maimari, C. Krasel, M. Bünemann, K. Lorenz:
    The N-termini of GRK2 and GRK3 simulate the stimulating effects of RKIP on β-adrenoceptors.
    Biochem. Biophys. Res. Commun. 520: 327-332 (2019)
    [ PDF ]
     

2020

  1. A. Gillis, A. B. Gondin, A. Kliewer, J. Sanchez, H. D. Lim, C. Alamein, P. Manandhar, M. Santiago, S. Fritzwanker, F. Schmidel, T. A. Katte, T. Reekie, N. L. Grimsey, M. Kassiou, B. Kellam, C. Krasel, M. L. Halls, M. Connor, J. R. Lane, S. Schulz, M. J. Christie, M. Canals:
    Low intrinsic efficacy for G protein activation can explain the improved side effect profiles of new opioid agonists.
    Sci. Signal. 13: eaaz3140 (2020)
    [ PDF ]
     
  2. S. Al-Sabah, L. Adi, M. Bünemann, C. Krasel:
    The effect of cell surface expression and linker sequence on the recruitment of arrestin to the GIP receptor.
    Front. Pharmacol. 11: 1271 (2020)
    [ PDF ]
     

2021

  1. A. Flöser, K. Becker, E. Kostenis, G. König, C. Krasel, P. Kolb, M. Bünemann:
    Disentangling bias between Gq, GRK2, and arrestin3 recruitment to the M3 muscarinic acetylcholine receptor.
    ELife 10: e58442 (2021).
    [ full text ]
     

2022

  1. N. Mösslein, L.-M. G. Pohle, A. Fuss, M. Bünemann, C. Krasel:
    Residency time of agonists does not affect the stability of GPCR-arrestin complexes.
    Br. J. Pharmacol. 179: 4107-4116 (2022).
    [ full text ]
     
  2. R. S. Haider, E. S. F. Matthees, J. Drube, M. Reichel, U. Zabel, A. Inoue, A. Chevigné, C. Krasel, X. Deupi, C. Hoffmann:
    β-arrestin1 and 2 exhibit distinct phosphorylation-dependent conformations when coupling to the same GPCR in living cells.
    Nat. Commun. 13: 5638 (2022).
    [ full text ]
     
  3. B. Al-Zaid, S. Chacko, C. I. Ezeamuzie, M. Bünemann, C. Krasel, T. Karimian, P. Lanzerstorfer, S. Al-Sabah:
    Differential effects of glucose-dependent insulinotropic polypeptide receptor/glucagon-like peptide-1 receptor heteromerization on cell signaling when expressed in HEK-293 cells.
    Pharmacol. Res. Perspect. 10: e01013 (2022).
    [ full text ]
     

2024

  1. T. Wang, J. Shao, S. Kumar, M. W. Alnouri, J. Carvalho, S. Günther, C. Krasel, K. T. Murphy, M. Bünemann, S. Offermanns, N. Wettschureck:
    Orphan GPCR GPRC5C facilitates angiotensin II-induced smooth muscle contraction.
    Circ. Res. 134: 1259-1275 (2024).
    [ full text ]
     
  2. S. Rinné, F. Schick, K. Vowinkel, S. Schütte, C. Krasel, S. Kauferstein, M. K.-H. Schäfer, A. K. Kiper, T. Müller, N. Decher:
    Potassium channel TASK-5 forms functional heterodimers with TASK-1 and TASK-3 to break its silence.
    Nat. Commun. 15: 7548 (2024).
    [ PDF ]
     

2025

  1. S. B. Kirchhofer, C. Kurz, L. Geier, A. L. Krett, C. Krasel, M. Bünemann:
    Dynamics of agonist-evoked opioid receptor activation revealed by FRET- and BRET-based opioid receptor conformation sensors.
    Commun. Biol. 8: 198 (2025).
    [ PDF ]
     

Reviews

  1. M. J. Lohse, K. Blüml, S. Danner, C. Krasel:
    Regulators of G-protein-mediated signaling.
    Biochem. Soc. Trans. 24, 975-980 (1996)
     
  2. M. J. Lohse, C. Krasel, R. Winstel, F. Mayor:
    G-protein-coupled receptor kinases.
    Kidney Int. 49, 1047-1052 (1996)
    [ PDF ]
     
  3. C. Krasel, J.-P. Vilardaga, M. Bünemann, M. J. Lohse:
    The kinetics of G protein-coupled receptor signaling and desensitization.
    Biochem. Soc. Trans. 32, 1029-1031 (2004).
    [ PDF ]
     
  4. C. Krasel, M. Bünemann:
    Opioid receptors: single molecule studies shed light on mechanisms of efficacy.
    Signal Transd. Targ. Ther. 9, 219 (2024).
    [ Article ]
     

Book chapters

  1. C. Krasel, M. J. Lohse:
    Signaling in the beta-adrenergic receptor system.
    In: Proc. XIVth Intern. Symp. Med. Chem., F. Awouters (ed.), Elsevier, Amsterdam 1997.
     
  2. M. J. Lohse, C. Krasel:
    G-protein-coupled receptor kinases.
    xPharm (executive editors: S. J. Enna, D. B. Bylund), Elsevier, New York 2004.
     
  3. C. Krasel, C. Hoffmann:
    Using intramolecular fluorescence resonance energy transfer to study receptor conformation.
    Chapter 7 in: D. Poyner, M. Wheatley (eds.): G-protein-coupled receptors.
    Wiley-Blackwell 2010. ISBN 978-0-47-074914-2 (print) 978-0-47.074921-0 (on-line).
    [ PDF ]
     
  4. C. Krasel, A. Rinne, M. Bünemann:
    Investigation of muscarinic receptors by fluorescent techniques.
    Chapter 8 in: J. Myslivecek, J. Jakubik (eds.): Muscarinic receptor: from structure to animal models.
    Neuromethods Vol. 107.
    Humana Press 2015. ISBN 978-1-4939-2857-6 (print) 978-1-4939-2858-3 (eBook).
    [ Book (PDF) ]
     
  5. C. Krasel, M. Bünemann:
    Investigation of muscarinic receptors by fluorescent techniques.
    Chapter 9 in: J. Myslivecek, J. Jakubik (eds.): Muscarinic receptor: from structure to animal models.
    Neuromethods Vol. 211.
    Humana Press 2024. ISBN 978-1-0716-4014-2 (print) 978-1-0716-4015-9 (eBook).
    [ Book (PDF) ]